Regulation of myosin light chain and phosphorylase phosphorylation in tracheal smooth muscle.
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منابع مشابه
Regulation of myosin light chain and phosphorylase phosphorylation in tracheal smooth muscle.
The activity of phosphorylase kinase and myosin light chain kinase can be regulated by calcium-calmodulin or cyclic AMP. Since phosphorylation of the 20,000dalton phosphorylatable light chain (P-light chain) of myosin may be involved in the regulation of contractile activity in smooth muscle, we examined the relationship between P-light chain phosphorylation, phosphorylase a formation, and isom...
متن کاملMyosin light chain kinase phosphorylation in tracheal smooth muscle.
Purified myosin light chain kinase from smooth muscle is phosphorylated by cyclic AMP-dependent protein kinase, protein kinase C, and the multifunctional calmodulin-dependent protein kinase II. Because phosphorylation in a specific site (site A) by any one of these kinases desensitizes myosin light chain kinase to activation by Ca2+/calmodulin, kinase phosphorylation could play an important rol...
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Myosin light chain kinase from smooth muscle has been shown to be phosphorylated by cyclic AMP-dependent protein kinase, which leads to a decrease in the affinity of the kinase for Ca2+ . calmodulin and, hence, a decrease in enzymatic activity. This event has been proposed as a mechanism for the relaxation of smooth muscle in response to increased intracellular concentrations of cyclic AMP. The...
متن کاملQuantitation of myosin light chain phosphorylation in intact smooth muscle.
An improved method for quantitating the extent of myosin light chain (P-LC) phosphorylation in small smooth muscle samples is described. Native myosin was isolated from other cellular proteins in a crude supernatant fraction prepared from a few milligrams of bovine tracheal smooth muscle by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium pyrophosphate (PPi). When potassium i...
متن کاملMyosin phosphorylation during contraction and relaxation of tracheal smooth muscle.
The role of myosin phosphorylation in regulating smooth muscle contraction has been investigated by quantitating the myosin phosphate content of tracheal smooth muscle frozen during contraction or relaxation. Myosin was purified from quick-frozen muscle samples with the aid of antibodies prepared against tracheal smooth muscle myosin, and the phosphate content was determined after separation of...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)65118-3